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Metabolomics (4-2):Metebolomics and Immunology Cultivation/新陈代谢组学之4-2

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发表于 2021-2-8 17:14:24 | 显示全部楼层 |阅读模式
This is the article 10-2 in the theme 'Environmental Physiology/环境生理学' of Journal of Environment and Health Science.

2021. Copyrights Certificate Registered by Brock Chain Technology: Certificate NO. (TTAS_S.0.2_63603937298403724634328462851118315019273479) ; Certificate Verification Web: https://ttas.ntsc.ac.cn

2016. Copyrights Register Information: The majority of these materials are registered as book '著作权人:刘焕;作品:《研究生文凭进展(第三版)》' 2016, which can be cataloged in National Copyright Database: http://qgzpdj.ccopyright.com.cn/

2016. 版权注册信息:本文大多数内容已经以图书形式登记注册在全国版权数据库,登记入库信息:著作权人:刘焕;作品:《研究生文凭进展(第三版)》 2016;可在全国版权登记数据库检索 http://qgzpdj.ccopyright.com.cn/

The formally published serials is the printing <Journal of Environment and Health Science (ISSN 2314-1628)>, and the serials NO. is the month/year when the materials accessible on this website, authorized by publisher;
正式发表的期刊是印刷版《环境与卫生科学杂志(ISSN 2314-1628)》,期刊期号为文章内容在本网站上网年/月,出版人许可自行正式发表。

Originality Certificate: The originality of text in English is 98% tested by Turnitin (International). The Turnitin Originality Report is attached in PDF summary.

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 楼主| 发表于 2021-2-8 17:14:49 | 显示全部楼层
Article 10-2. The Experiment Procedure for Blood Cell Cultivation in Biophysical Simulation/生物物理实验中血细胞培养方法
Author: Liu Huan, MSc (First Class Honours), The University of Auckland.

Published after graduation on 11/01/2016, Revised on 08/02/2021.


The blood samples of a rat is abstracted and divided into two samples for the bio-signal simulation:
1.There are two kinds of cultivation conditions simulated in Lab for cell division: one is the ‘comfortable’ condition (Sample 1); the other is under electromagnetism simulation for cell cultivation (Sample 2); the cell samples are collected after sufficient cell division (Ten generations).

2.After sufficient cell division process, the electromagnetism simulation stops. Then both sample 1 and sample 2 are separately transferred into the simulation process of physiological saline: cells are cultivated individually in different concentrations of physiological saline in Lab, and different cell environment (salinity stress of cell environment or ‘thirsty’ simulation) are labeled as T1, T2, ..., Tn.

3.Metabolomics tests are conducted (listed by the article 1 of this journal) in cell samples after simulation process of physiological saline, T1, T2, ..., Tn, respectively, resulting in different zymograms as: M1, M2, ..., Mn.

The other procedures are the same as described above.
However, for the comprehensive assessment of immunology in host cells, the simulation process of physiological saline is replaced by the invasion simulation caused by different families of bacteria (or virus):
The blood samples of a rat is abstracted and divided into two samples for the bio-signal simulation:
1.There are two kinds of cultivation conditions simulated in Lab for cell division: one is the ‘comfortable’ condition (Sample 1); the other is under electromagnetism simulation for cell cultivation (Sample 2); the cell samples are collected after sufficient cell division (Ten generations).

2.After sufficient cell division process, the electromagnetism simulation stops. Then both sample 1 and sample 2 are separately transferred into the simulation process of bacteria (or virus) invasion: cells are cultivated individually and independently during the simulation of different families of bacteria (or virus) in Lab, and the invasion simulation process of different bacteria (or virus) families are labeled as T1, T2, ..., Tn.

3.Metabolomics tests are conducted (listed by the article 1 of journal) in cell samples after simulation process  of bacteria  (or  virus) invasion, T1,  T2, ..., Tn, respectively, resulting in different zymograms as: M1, M2, ..., Mn.

The other procedures are the same as described above. This comprehensive assessment of immunology is closer to the real situation of disease caused by multiple species of bacteria, as described by the chapter 8 of this book. Even if the pathology  of host cells (such as cancerous blood cells of rat) is not caused by multiple species of invasive virus or bacteria (and by one species only), the invasive virus or bacteria of the same genetic strain also evolves into various phenotypes in host body, which reflects the significance of comprehensive assessment of immunology.

Please note: if all the blood cells have been ‘eaten’ up (or no cell division rate) by a strain of bacteria during invasion simulation, then the value of this zymogram can be counted as zero for subsequent matrix calculation.

For the comprehensive assessment of immunology in host cells caused by the invasive virus or bacteria of the same genetic strain with different phenotypes:

The blood samples of a rat is abstracted and divided into two samples for the bio-signal simulation:
1.There are two kinds of cultivation conditions simulated in Lab for cell division: one is the ‘comfortable’ condition (Sample 1); the other is under electromagnetism simulation for cell cultivation (Sample 2); the cell samples are collected after sufficient cell division (Ten generations).
2.After sufficient cell division process, the electromagnetism simulation stops. Then both sample 1 and sample 2 are separately transferred into the simulation process of bacteria (or virus) invasion of the same genetic strain with different phenotypes: cells are cultivated individually and independently during the invasive simulation by different phenotypes of the same genetic bacteria (or virus) in Lab, and the invasion simulation process by different phenotypes of the same genetic bacteria (or virus) are labeled as T1, T2, ..., Tn.

3.Metabolomics tests are conducted (listed by article 1 of this journal) in cell samples after simulation process  of bacteria (or  virus) invasion, T1,  T2, ..., Tn, respectively, resulting in different zymograms as: M1, M2, ..., Mn.

The other procedures are the same as described above. This electromagnetism simulation can be either constant electromagnetism fields or time-varying electromagnetic waves, which are further discussed later.




Conclusion:
The comprehensive assessment of immunology in host cells also provides indicators of training host cells by adjusting the parameters of biophysical simulation, once the specific zymograms, indicating the immunology against the specific invasive bacteria or virus (or the specific phenotype of an invasive pathogen), are identified by the methods presented in the other article of this journal. However, the higher dynamics, the better immunology against various pathogen species (or various phenotypes of a pathogen genotype).










This is the revised materials in book “Proceedings for Degree of Postgraduate Diploma in Environmental Science (3rd Edition).” Published in 2016. The ‘chapter’ content mentioned in this article is in previous book. Firstly Revised on 05/01/2021; Secondly Revised on 08/02/2021.

References:
All the science terms in English of this journal source from Wikipedia:
https://encyclopedia.thefreedictionary.com/;
本文所有中文科学专业术语引用自百度百科 https://baike.baidu.com/.
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