The Identification of Pathogen Phenotype for Biophysical Simulation

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This journal article is previously published as: Liu Huan. (2021). Article 10-3. The Identification of Pathogen Phenotype for Biophysical Simulation. Journal of Environment and Health Science (ISSN 2314-1628), 2021(02)., which is converted into Journal of Biological Sciences (ISSN 2958-4035). Both Journals belong to the same publisher, Liu Huan. The previous journal article is closed to the public, but the previous reference is still valid.

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Cited as: DOI:10.58473/JBS0010

ORCID: https://orcid.org/0000-0003-4881-8509

Article 6-3. The Identification of Pathogen Phenotype for Biophysical Simulation /生物物理模拟试验中病原体表现型的确定方法

Author: Liu Huan (1983-), Master of Science (First Class Honours), The University of Auckland.

Step 1. The host cells of the same genetic strain (such as the blood cells of rat) are abstracted, which are divided  into  several  cell  samples,  and  labeled  as  S1, S2, S3, Sn;

Step 2. The simulation of a specific virus (or bacteria) invasion targeting the host cells is conducted in Lab, immediately after host cells are abstracted from host body;

Step 3. The samples of host cells with apparent antibiotics are identified, as described by the other article of this journal; and the samples of host cells without apparent antibiotics are also continuously observed until they are ‘eaten up’ by the specific invasive pathogen;

Step 4. The separation of virus from each sample of host cell without apparent antibiotics are conducted independently in Lab, and the metabolomics test is conducted in each virus sample [1];

Objective:

The different phenotypes of an invasive virus (or bacteria) strain are identified, and  the biochemistry dynamics of this invasive virus strain is calculated. The result of biochemistry dynamics calculation of host cells discussed in my previous article, which indicates that which enzyme species underlie the synthesis of antibiotics in host cells, helps to determine the range of bio-physical training parameters to enhance the comprehensive immunology of host cells. However, the identification of the invasive phenotype of pathogen is more exactly targeting the specificity of host-invasive interaction.

Please note: the simulation of a specific virus (or bacteria) invasion targeting the host cells should be conducted immediately after host cells are abstracted from host body, otherwise the uniform cell cultivation in Lab lead to the homogeneity of host cells, so that different phenotypes of an invasive pathogen can be hardly detected.

Because the virus sample for invasion simulation is cultivated in Lab, which is the uniform phenotype, the samples of host cells with apparent antibiotics usually show specific zymograms correspondingly to the specific invasive virus. However, if virus samples, which are separated from host cell without apparent antibiotics after step 3, re-invade the host cells with apparent antibiotics identified in step 3, virus infection would occur, due to the evolution of new virus phenotypes.

This is the revised materials in book “Proceedings for Degree of Postgraduate Diploma in Environmental Science (3rd Edition).” Published in 2016. The ‘chapter’ content mentioned in this article is in previous book. Firstly Revised on 05/01/2021; Secondly Revised on 08/02/2021; Thirdly Revised on 26/09/2021; Fourthly Revised on 25/12/2021. This journal article is previously published as: Liu Huan. (2021). Article 10-3. The Identification of Pathogen Phenotype for Biophysical Simulation. Journal of Environment and Health Science (ISSN 2314-1628), 2021(02)., which is converted into Journal of Biological Sciences (ISSN 2958-4035). Both Journals belong to the same publisher, Liu Huan. The previous journal article is closed to the public, but the previous reference is still valid.  

References:

[1].Liu Huan. Metabolomics (1) --- The Systematic Chemistry Fingerprints Between Genotype and Phenotype and its Application on the Conservation Genetics. Journal of Environment and Health Science. Feb. 2021.